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mouse anti isl1 2 antibody  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mouse anti isl1 2 antibody
    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number <t>of</t> <t>ISL1/2</t> + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.
    Mouse Anti Isl1 2 Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isl1+2+mouse/anti-Islet-1+%26+Islet-2+homeobox/bio_rxiv__64898__2026__05__07__723626-324-50-54
    Average 96 stars, based on 279 article reviews
    mouse anti isl1 2 antibody - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Engineering a pacemaker-driven human mini-heart guided by spatial and single cell multi-omics of sinoatrial node development"

    Article Title: Engineering a pacemaker-driven human mini-heart guided by spatial and single cell multi-omics of sinoatrial node development

    Journal: bioRxiv

    doi: 10.64898/2026.05.07.723626

    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number of ISL1/2 + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.
    Figure Legend Snippet: a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number of ISL1/2 + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.

    Techniques Used: Fluorescence, Control, Staining

    Related Articles

    Droplet Countercurrent Chromatography:

    Article Title: Nck2 is essential for limb trajectory selection by spinal motor axons.
    Article Snippet: .. Received: Oct 14, 2017; Revised: Apr 23, 2018; Accepted: May 12, 2018 D ev el op m en ta l D yn am ic s D ev el op m en ta l D yn am ic s Table 1: Antibodies and Fc reagents used Antigen / pure protein Source species Dilution Source/reference EphA4 Rabbit 1:500 Santa Cruz EphB1 Goat 1:500 Santa Cruz Nck2 Rabbit 1:500 Merck neurofilament ouse (2H3) 1:100 DSHB Mouse (3A10) 1:100 DSHB Isl1/2 Mouse 1:100 DSHB Lim1/2 Mouse 1:100 DSHB HRP Goat 1:2000 Roche Foxp1 Rabbit 1:1000 Abcam DCC Goat 1:500 R&D system GFP Guinea pig 1:1000 AbD Serotec GFP Rabbit 1/1000 Molecular Probes ephrin-A5-Fc Human 10 μg/mL R&D system ephrin-B2-Fc Mouse 10 μg/mL R&D system Semaphorin 3F-Fc Mouse 10 μg/mL R&D system Netrin-1 Mouse 250 ng/mL R&D system Fc Human 10 μg/mL R&D system anti-Fc Mouse 1:4 mass ratio to ephrin Sigma Goat 1:4 mass ratio to ephrin Sigma This article is protected by copyright. ..

    other:

    Article Title: Vav2 is required for Netrin-1 receptor-class-specific spinal motor axon guidance.
    Article Snippet: The Ph.D.. Program for Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University and National Health Research Institutes, Taipei, Taiwan Department of Neurosurgery, Taipei Medical University Hospital, Taipei, Taiwan Taipei Neuroscience Institute, Taipei Medical University, Taipei, Taiwan Graduate Institute of Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan Division of Neurosurgery, Department of Surgery, Min-Sheng General Hospital, Taoyuan, Taiwan Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan Research Center of Neuroscience, Taipei Medical University, Taipei, Taiwan TMU Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei, Taiwan Department of Neurosurgery, Cheng Hsin General Hospital, Taipei, Taiwan Ph.D.. Program of Electrical and Communications Engineering, Feng Chia University, Taichung, Taiwan

    In Vitro:

    Article Title: Cx43 hemichannels contribute to astrocyte-mediated toxicity in sporadic and familial ALS
    Article Snippet: .. Primary antibodies used for in vitro and in vivo immunostaining, western blot (WB), Antibody Species Staining dilution WB dilution Company (cat no) Cx43 Rabbit 1:500 1:5000 Millipore Sigma (C6219) GFAP Chicken 1:200 - Millipore sigma (AB5541) GFAP Mouse 1:200 1:5000 Millipore Sigma (MAB360) S100β Rabbit 1:500 - Millipore Sigma (ABN59) Tuj1 Rabbit 1:500 - Millipore Sigma (AB9354) ChAT Goat 1:100 - Millipore Sigma (AB144P) Isl1/2 Mouse 1:50 - DSHB (39.4D5, concentrate) Iba-1 Rabbit 1:100 - Wako (019-19741) Cx30 Rabbit 1:500 - Invitrogen (700258) hSOD1 Rabbit - 1:2000 Chemicon (AB5480) GLT-1 Rabbit 1:500 1:1000 (*) GLAST (EAAT1) Rabbit - 1:100 (*) GAPDH Mouse - 1:5000 Millipore Sigma (G8795) Actin Rabbit - 1:1000 Cell Signaling (4967) (*) Kindly provided by Dr Jeffrey D. Rothstein’s Lab ..

    In Vivo:

    Article Title: Cx43 hemichannels contribute to astrocyte-mediated toxicity in sporadic and familial ALS
    Article Snippet: .. Primary antibodies used for in vitro and in vivo immunostaining, western blot (WB), Antibody Species Staining dilution WB dilution Company (cat no) Cx43 Rabbit 1:500 1:5000 Millipore Sigma (C6219) GFAP Chicken 1:200 - Millipore sigma (AB5541) GFAP Mouse 1:200 1:5000 Millipore Sigma (MAB360) S100β Rabbit 1:500 - Millipore Sigma (ABN59) Tuj1 Rabbit 1:500 - Millipore Sigma (AB9354) ChAT Goat 1:100 - Millipore Sigma (AB144P) Isl1/2 Mouse 1:50 - DSHB (39.4D5, concentrate) Iba-1 Rabbit 1:100 - Wako (019-19741) Cx30 Rabbit 1:500 - Invitrogen (700258) hSOD1 Rabbit - 1:2000 Chemicon (AB5480) GLT-1 Rabbit 1:500 1:1000 (*) GLAST (EAAT1) Rabbit - 1:100 (*) GAPDH Mouse - 1:5000 Millipore Sigma (G8795) Actin Rabbit - 1:1000 Cell Signaling (4967) (*) Kindly provided by Dr Jeffrey D. Rothstein’s Lab ..

    Immunostaining:

    Article Title: Cx43 hemichannels contribute to astrocyte-mediated toxicity in sporadic and familial ALS
    Article Snippet: .. Primary antibodies used for in vitro and in vivo immunostaining, western blot (WB), Antibody Species Staining dilution WB dilution Company (cat no) Cx43 Rabbit 1:500 1:5000 Millipore Sigma (C6219) GFAP Chicken 1:200 - Millipore sigma (AB5541) GFAP Mouse 1:200 1:5000 Millipore Sigma (MAB360) S100β Rabbit 1:500 - Millipore Sigma (ABN59) Tuj1 Rabbit 1:500 - Millipore Sigma (AB9354) ChAT Goat 1:100 - Millipore Sigma (AB144P) Isl1/2 Mouse 1:50 - DSHB (39.4D5, concentrate) Iba-1 Rabbit 1:100 - Wako (019-19741) Cx30 Rabbit 1:500 - Invitrogen (700258) hSOD1 Rabbit - 1:2000 Chemicon (AB5480) GLT-1 Rabbit 1:500 1:1000 (*) GLAST (EAAT1) Rabbit - 1:100 (*) GAPDH Mouse - 1:5000 Millipore Sigma (G8795) Actin Rabbit - 1:1000 Cell Signaling (4967) (*) Kindly provided by Dr Jeffrey D. Rothstein’s Lab ..

    Western Blot:

    Article Title: Cx43 hemichannels contribute to astrocyte-mediated toxicity in sporadic and familial ALS
    Article Snippet: .. Primary antibodies used for in vitro and in vivo immunostaining, western blot (WB), Antibody Species Staining dilution WB dilution Company (cat no) Cx43 Rabbit 1:500 1:5000 Millipore Sigma (C6219) GFAP Chicken 1:200 - Millipore sigma (AB5541) GFAP Mouse 1:200 1:5000 Millipore Sigma (MAB360) S100β Rabbit 1:500 - Millipore Sigma (ABN59) Tuj1 Rabbit 1:500 - Millipore Sigma (AB9354) ChAT Goat 1:100 - Millipore Sigma (AB144P) Isl1/2 Mouse 1:50 - DSHB (39.4D5, concentrate) Iba-1 Rabbit 1:100 - Wako (019-19741) Cx30 Rabbit 1:500 - Invitrogen (700258) hSOD1 Rabbit - 1:2000 Chemicon (AB5480) GLT-1 Rabbit 1:500 1:1000 (*) GLAST (EAAT1) Rabbit - 1:100 (*) GAPDH Mouse - 1:5000 Millipore Sigma (G8795) Actin Rabbit - 1:1000 Cell Signaling (4967) (*) Kindly provided by Dr Jeffrey D. Rothstein’s Lab ..

    Staining:

    Article Title: Cx43 hemichannels contribute to astrocyte-mediated toxicity in sporadic and familial ALS
    Article Snippet: .. Primary antibodies used for in vitro and in vivo immunostaining, western blot (WB), Antibody Species Staining dilution WB dilution Company (cat no) Cx43 Rabbit 1:500 1:5000 Millipore Sigma (C6219) GFAP Chicken 1:200 - Millipore sigma (AB5541) GFAP Mouse 1:200 1:5000 Millipore Sigma (MAB360) S100β Rabbit 1:500 - Millipore Sigma (ABN59) Tuj1 Rabbit 1:500 - Millipore Sigma (AB9354) ChAT Goat 1:100 - Millipore Sigma (AB144P) Isl1/2 Mouse 1:50 - DSHB (39.4D5, concentrate) Iba-1 Rabbit 1:100 - Wako (019-19741) Cx30 Rabbit 1:500 - Invitrogen (700258) hSOD1 Rabbit - 1:2000 Chemicon (AB5480) GLT-1 Rabbit 1:500 1:1000 (*) GLAST (EAAT1) Rabbit - 1:100 (*) GAPDH Mouse - 1:5000 Millipore Sigma (G8795) Actin Rabbit - 1:1000 Cell Signaling (4967) (*) Kindly provided by Dr Jeffrey D. Rothstein’s Lab ..



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    Developmental Studies Hybridoma Bank mouse anti isl1 2 antibody
    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number <t>of</t> <t>ISL1/2</t> + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.
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    Developmental Studies Hybridoma Bank mouse anti isl1 2 39 4 d5
    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number <t>of</t> <t>ISL1/2</t> + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.
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    Developmental Studies Hybridoma Bank mouse anti isl1 2
    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number <t>of</t> <t>ISL1/2</t> + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.
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    Developmental Studies Hybridoma Bank mouse anti isl1 2 monoclonal antibody
    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number <t>of</t> <t>ISL1/2</t> + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.
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    Image Search Results


    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number of ISL1/2 + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.

    Journal: bioRxiv

    Article Title: Engineering a pacemaker-driven human mini-heart guided by spatial and single cell multi-omics of sinoatrial node development

    doi: 10.64898/2026.05.07.723626

    Figure Lengend Snippet: a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number of ISL1/2 + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.

    Article Snippet: At 54 hpf larvae were fixed in 2% PFA for 2 hours at room temperature, washed extensively in PBS with 0.3% TritonX-100 (PBX), incubated in blocking buffer (BB: PBX, 0.5% BSA, 10% goat serum) for 2 hours at room temperature, incubated overnight at 4°C in BB containing 1/50 dilution of mouse anti-ISL1/2 antibody (Iowa Developmental Studies Hybridoma Bank #39.4D5), washed extensively with PBX, incubated at room temperature for 2 hours in BB with a 1/200 dilution of goat anti-mouse IgG2b Alexa Fluor568 (Invitrogen #A-21144), washed extensively with PBX, and mounted in 1:1 PBS:glycerol for imaging on a Zeiss LSM800 confocal microscope.

    Techniques: Fluorescence, Control, Staining